Nested PCR检测微量隐孢子虫卵囊

根据隐孢子虫18SrDNA序列，设计出隐孢子虫属和鼠隐孢子虫种的特异性引物，进行PCR和Nested PCR反应，先后分剐扩增出1条540bp和1条250bp的条带。研究表明，NestedPCR具有高度的特异性和敏感性。应用NestedPCR可检测1～10个鼠隐孢子虫卵囊，其敏感性是饱和蔗糖漂浮法的10^5倍以上。     

马尾松毛虫危害预报的研究

文章对在马尾松毛虫危害预报中，应用马尔可夫链方法并结合Lotka-Volterra生态数学模型进行预测预报进行了论述。     

稻田群落食物链结构优化与生态经济效益分析

双季连作稻田引入萍，鱼，螺组成一定的群落食物链后，稻田的系统生产力显著提高。对稻田绿萍的消长因素和萍－鱼食物链行为进行计算模拟的结果表明：在纯稻田的稻－萍－鱼系统放养草鱼夏花的数量以４５０００尾／ｈｍ￣２为宜。田塘式稻－萍─鱼夏花草鱼苗可放养７５０００尾／ｈｍ￣２．稻田组建群落食物链对水稻生长无明显不利影响，且有增产作用。     

具有函数放养率或捕获率n步食物链生态系统的稳定性

运用Ｌｉａｐｕｎｏｖ第二方法研究具有函数放养率或捕获率ｎ步食物链生态系统的稳定性，由此得到其全局稳定，大范围稳定，不稳定的判定条件，是很有理论和实践意义的。     

基于CSMA/CD/RR协议的有限缓存排队系统性能分析

提出了一种具有动态分配重传策略的CSMA/CD协议，并利用二维马尔可夫链分析了有限缓冲CSMA/CD/RR协议的性能特性，仿真实验表明，与CSMA/CD协议相比，CSMA/CD/RR协议很好地解决了高负载时的拥塞问题。     

Restricted expansion of T cell receptor (TCR)Vβ genes in leukemic patients subjected to allogeneic hematopoeitic stem cell transplantation

AIM: To investigate restricted expansion of TCR Vβ gene repertoire in patients with leukemia following allogeneic hematopoietic stem cell transplantation. METHODS: TCR Vβ subfamily genes in peripheral blood mononuclear cells from 7 cases of leukemia was amplified using RT-PCR. RESULTS: Only two-eight fragments of Vβ genes were detected in samples from these patients, and the detected fragments are different in different patients. CONCLUSION: TCR complexes were abnormal in all patients, part of the genes were seletively expansed and part of them were suppressed after transplantation.     

Contribution of the polymerization of protein by disulfide bonding to increased gel strength of walleye pollack surimi gel with preheating time

ABSTRACT: To clarify the contribution of polymerization of myosin heavy chain (MHC) by disulfide bonding to increased gel strength of cooked gel via preheating, the pastes of walleye pollack surimi (SS and C grades) were preheated at 25°C and 40°C for a variety of hours prior to heating at 80°C for 20 min. Sodium dodecyl sulfate–polyacrylamide gel electrophoresis (SDS-PAGE) patterns of cooked gels were analyzed with and without reducing the samples, which were solubilized in 8 M urea–2% SDS solution. The formation of polymers by disulfide bonding in cooked gels was almost constant in each of the SS and C grade surimi gels despite the period of preheating. Therefore, it was suggested that polymerization by disulfide bonding occurred during cooking at 80°C and not during preheating.     

Inhibiting effect of polymerization and degradation of myosin heavy chain during preheating at 30°C and 50°C on the gel-forming ability of walleye pollack surimi

ABSTRACT: To confirm the contribution of polymerization and degradation of myosin heavy chain (MHC) during preheating to the gel-forming ability of fish meat paste, walleye pollack surimi paste was preheated at 30°C and 50°C prior to heating at 80°C in the presence of various inhibitors. At 30°C, ethyleneglycol bis(2-aminoethyl ether) -N,N,N ', N '-tetraacetic acid (EGTA) and ethylenediaminetetraacitic acid (EDTA) inhibited gel formation as well as the polymerization of MHC, whereas dithiothreitol (DTT) and leupeptin promoted gel formation, which was accompanied by the enhancement of MHC polymerization and decreased MHC degradation, respectively. At 50°C, leupeptin inhibited MHC degradation and improved gel strength, whereas EGTA, EDTA and DTT had no effect on MHC polymerization and degradation and did not affect gel formation. The results demonstrate that the gel strength of cooked gel (80°C) is not affected by preheating at 30°C and 50°C and does not inhibit polymerization and degradation. Results suggest that the gel strength of cooked gel is dependent on the polymerization and degradation of MHC during preheating.     

锯链传动链片的有限元分析

用有限元法对锯链的传动链片进行了应力分析，结果表明：危险剖面上的应力分布是不均匀的，在运转中传动链片所受的应力为变应力，按传统的方法仅计算静强度是不够的，要提高锯链的使用寿命，必须提高锯链零件的疲劳强度。     

锯链连接片偏磨原因的受力分析及解决途径的研究

通过对国内外万能锯链负荷工作时的受力分析,发现现有万能锯链的切齿组件的设计几何参数不尽合理。因而,造成正压合力集中,促使连接片偏磨,缩短了锯链寿命。同时,偏磨后的切齿组件切削功能恶化,影响了链锯机械性能的发挥。为此,重新设计了切齿组件,使正压合力趋向多点均匀分布。经生产、台架试验证明,新型锯链的使用寿命,是国产锯链的2倍左右,是斯蒂尔锯链的1.5倍;切削效率比国产锯链提高35—116.7％,比奥利根锯链提高3—4％。同时,切齿组件在整个生产过程中,始终保持着良好的切削状态和原设计的主要几何参数。证明“正压合力多点均匀分布”的设计理论是克服连接片偏磨的可靠途径。